Journal: Cancer Science

Article Title: Resveratrol enhances radiation sensitivity in prostate cancer by inhibiting cell proliferation and promoting cell senescence and apoptosis

doi: 10.1111/j.1349-7006.2012.02272.x

Figure Lengend Snippet: Effect of radiation therapy/resveratrol (XRT/RSV) on the expression of pro‐ and antiproliferative molecules evaluated by immunohistochemistry and Western blot. (a) Immunohistochemistry for p21, p27, and p53 in PC‐3 cells treated with or without XRT (8 Gy) in the presence or absence of RSV (50 μM). Original magnification, ×400. (b) Relative immunostaining intensity for p21, p27, and p53 in five to six randomly selected high power fields of three slides from each group analyzed by MetaMorph software. Results are expressed as the average integrated immunostaining intensity of three slides + SEM relative to that in control cells. (c) Protein expression levels of p21, p27, and p53 by Western blot analysis; 30 μg protein was loaded in each lane. *P < 0.05, significant difference in immunostaining intensity or protein expression level in the groups treated with XRT or XRT/RSV versus controls. #P < 0.05, significant difference in immunostaining intensity or protein expression level between the group treated with XRT/RSV versus the group treated with XRT alone. Figures shown are representative of two or three independent experiments.

Article Snippet: 21 To quantify the number of apoptotic cells, all cells in five or six randomly selected high power fields (magnification, ×400) were manually counted using MetaMorph image analysis software.

Techniques: Expressing, Immunohistochemistry, Western Blot, Immunostaining, Software, Control

Journal: Cancer Science

Article Title: Resveratrol enhances radiation sensitivity in prostate cancer by inhibiting cell proliferation and promoting cell senescence and apoptosis

doi: 10.1111/j.1349-7006.2012.02272.x

Figure Lengend Snippet: Effect of radiation therapy/resveratrol (XRT/RSV) on the expression of p‐AKT and p‐H2A.X evaluated by immunohistochemistry and Western blot analysis. (a) Immunohistochemistry for p‐AKT and p‐H2A.X in PC‐3 cells treated with or without XRT (8 Gy) in the presence or absence of RSV (50 μM). Original magnification, ×400. (b) Relative immunostaining intensity for p‐AKT and p‐H2A.X was analyzed by MetaMorph software. Results are expressed as the average integrated immunostaining intensity of three slides + SEM relative to that in control cells. (c) Shown are Western blot results for the protein expression levels of p‐AKT and p‐H2A.X; 30 μg protein was loaded in each lane. *P < 0.05, significant difference in immunostaining intensity or protein expression level in the groups treated with XRT or XRT/RSV versus controls. #P < 0.05, significant difference in immunostaining intensity or protein expression level between the group treated with XRT/RSV versus the group treated with XRT alone. Figures are representative of two or three independent experiments.

Article Snippet: 21 To quantify the number of apoptotic cells, all cells in five or six randomly selected high power fields (magnification, ×400) were manually counted using MetaMorph image analysis software.

Techniques: Expressing, Immunohistochemistry, Western Blot, Immunostaining, Software, Control